Comparison between Plasma Glucose Concentration in Fluoride Oxalate and Ethylene Di-amine Tetra Acetate (EDTA) Anticoagulants

Abstract

This study was carried out in Al-Madeina Al-Monwara clinic in AL-jreaf Shrege during period 2013-2014, to measure glucose concentration using Fluoride Oxalate anticoagulant compared to EDTA anticoagulant during different periods of time (immediately after collection (0), after 2 hours and after 4 hours); to determine the effect of time in glucose concentration in each anticoagulant. Fifty (50) subjects with different age groups were informed about the study and agreed for the participation. Five ml of venous blood sample was collected in both anticoagulants 2.5 ml for each sample. Plasma sample was separated using centrifuge, the concentration of glucose was determined during different periods using Digital Colorimeter. The results obtained and analyzed using software program (SPSS) to give means reduction ± SD of concentrations in both anticoagulants. The results showed that the differences between glucose concentrations in Fluoride Oxalate anticoagulant compared to EDTA anticoagulant is insignificant, while the difference between glucose concentrations during different periods of time in each anticoagulant is significantly different. An mean variation of glucose concentrations between (0- 2) hours is 11.7 mg/dl and 14.2 mg/dl in Fluoride Oxalate and EDTA anticoagulants respectively, between (2-4) hour is 13.8 mg/dl and 13.7 mg/dl respectively, and between (0-4) hours is 25.6 mg/dl and 27.8 mg/dl respectively.