Abstract:
This study was conducted in the Nutrition laboratory, College
of science and technology of Animal Production, Sudan University
of Science and Technology, during April - June 2009. The aim of
IIIthe study is to investigate the effects of enzyme concentration and
incubation period on gelatin extraction and the possibility of
gelatin extraction from cattle hides by using different concentration
of pancreatic alkaline enzyme containing trypsin and pepsin (Rock
enzyme 7500-L) for different incubation periods. Pieces of
Limed–fleshed cattle hides weighing 5kgs were used in this study.
They were cut into small pieces each 5cm2 delimed, degreased and
washed. The samples were treated with different concentration of
enzyme (0%, 1%, and 2%) for three different incubation periods
(three, six and nine hours). The extraction continued for six hours
in Water Bath at 70Co. The yield, color and pH of gelatin were
measured. The study showed that the concentration of the enzyme
has highly significant effect (**) at (p<0.01) on gelatin yield. The
percentages of gelatin yield of the first extraction at 0%, 1% and
2% was found to be 4.33%, 6.75% and 8.6% for three hours , 4.2
%, 6.61% and 8.11% for six hours , 4.31%, 7.3% and 9.21 % for
nine hours respectively . Higher yields of gelatin extraction were
obtained by using 2% of enzyme. In this study the color of gelatin
extracted at 0%, 1% and 2% enzyme concentration were very light
creamy, light creamy and creamy, while the pH of gelatin extracted
at the same concentration were in average 6.98, 6.96 and 6.98
respectively.
