Abstract:
This study was cross sectional hospital based to detect the mutation of
WT1 gene in Sudanese patients with acute myeloid leukemia, this study
was carried out in Radiation and Isotopes Center in Khartoum ,during the
period March 2012 to Feb Dec 2013.
Fifty one Sudanese patients with Acute Myeloid Leukemia were
informed about the study and the agreement for participation was
obtained, twenty six were male and twenty five were female, their ages
range between 10 – 70 years old.
5.0 ml of a venous blood were collected in Ethylene Diamine Tetra
Acetic acid container, Deoxyribonucleic Acid extraction was done in all
patient samples using the salting out method and the quality of extracted
DNA were checked on 1% agrose gel by electrophoresis, and then
Polymerase Chain Reaction amplification for exon 7 of Williams's tumor
1 gene was done using the specific primers from the published data and
the Polymerase Chain Reaction product was 214 bp. For mutation
detection restriction fragment length polymorphism was done in all
Polymerase Chain Reaction products from the patient samples using the
restriction enzyme AflIII.
The mean age of study population was (2.51), and tribal group
distribution among the study population were 38 (74.5%) from Afro
Asiatic, 3 (5.9%) from Nilo Saharan and 10 (19.6%) from Niger
Kordofanian tribal group. genotyping distribution among the study
population were 27 (52.9%) were normal (A/A) of Williams's tumor1
mutation, 22 (43.1%) were heterozygous (A/G) of Williams's tumor 1
gene mutation and only 2 (3.9%) of patients were homozygous (G/G) of
Williams's tumor 1 gene mutation
In this study we have found the mutation of Williams's tumor 1 gene in
24 of 51 (47.1%), Acute Myeloid Leukemia cases, This mutation
frequency is not equivalent to the previous studies of Williams's tumor 1
gene mutation in Acute Myeloid Leukemia, Subsequent studies revealed
that Williams's tumor 1 is mutant in approximately 10 - 15% of primary.
In conclusion, the result suggest a possible role of Williams's tumor 1
gene mutation in the development of Acute Myeloid Leukemia in
Sudanese and that is because of the heterozygous nature of the mutation ,
this gene act as an oncogene that inherited as dominant allele (only one
mutant allele could causes the disease).
