Abstract:
The main objective of this study was to isolate the possible bacterial
pathogens from the urinary tract of diabetic and non-diabetic patients (as
control). A total of hundred specimens (n=100) were collected from Jabir
Abuliz Specialized Center for diabetic patients, Khartoum North
Teaching Hospital and Police Hospital. Samples were collected from
both males and females at different ages and then cultured and incubated
at 370C for overnight. Isolated bacteria were identified by Gram stain
and biochemical tests. The most common isolated bacteria among the
diabetic Patients were Escherichia coli (16%), followed by Klebsiella
pneumoniae
(10%),
Staphylococcus
aureus
(8%)
Pseudomonas
aeruginosa (6%), Staphylococcus saprophyticus (4%), Proteus mirabilis
(2%) and Enterococcus faecalis (2%),
E. coli were the most prevalent bacteria (42%) isolated among non-
diabetic patients, followed by S. aureus (12%), K. pneumoniae (8%), P.
aeruginosa (6%) and P. mirabilis (4%). The study showed that among
diabetic patients, infections of females were fairly high (70.8%)
compared to male patients (29.1%). However, prevalence of infection
was significantly higher (P < 0.05) among non- diabetic females (75%)
than diabetic females. Most isolated Gram – negative bacteria were
sensitive to trimethoprim, ciprofloxacin, nitrofourantoin, nalidixic acid,
ceftazidime and ampicillin except P. aeruginosa, P. mirabilis, which
were resistant to ampicillin among diabetic patients. Furthermore, the
most isolated Gram –positive bacteria were sensitive to gentamicin,
vancomycin and penicillin except S. saprophyticus and E. faecalis
among diabetic patients and S. aureus among non-diabetic patients.
