Appropriateness of Clinical Laboratory Photometers Quality Requirements

Abstract

The aim of this study is to evaluate the efficiency and calibration of laboratories, spectrophotometers according to the specification (ISO 15189) and depending on the conditions (4.2.5 and 5.3.2) included in the items of laboratory equipment. At the beginning, structured questioner was conducted to assess the technician’s awareness of instruments calibration. The practical results were analyzed by using Statistical package for social service (SPSS 15). The study was applied on 40 spectrophotometers, which were calibrated by using special tools to show: the efficiency of filters to light, the severity of an accurate light degree and the ability of instrument to reach a temperature between (25±0.2°C and 37± 0.2°C) and which has a direct impact on clinical readings of instruments. The analysis procedure showed that a significant improvement (by 100% and p > 0.05) in results after calibration, which was evaluated by using the probability of obtaining a test statistic (p-value) in T test, for example the obtained results before calibration: in filter 340 nm(P < 0.012) with percentage average 43.9%, the temperature within range 25±0.2°C (P < 0.001) with percentage average 8.3%, the temperature within 37± 0.2°C (P < 0.001) with percentage average 0.00%, and the degree of an accurate lamp without a filter 172 nA (P < 0.001) with percentage average 30.8%, which leads to conclude that a process of calibration of instruments must be performed at frequent intervals due to its importance in enhancement the clinical results.