Abstract:
This study has been done on two groups of individuals for
the isolation of Haemophilus influenzae from sputum. The first
group of study include 50 individual patients presented with
pneumonia, whilst the second group included tuberculosis patients,
and those who are suspected to have tuberculosis (359) individual;
The age range in both groups lie between (3-73year). Collection of
the specimens was done within eight months from (April-Decimber)
through 2003 from Khartoum state, and Elobied city in North
Kordofan state.
Nine strains of Haemophilus influenzae were isolated from
the specimens. The percentage of Haemophilus influenzae isolated
from the specimens was 2.2%. The percentage of ß-Lactamase
producing strains of Haemophilus influenzae type b, was found to be
42.9%. The percentage of type b Haemophilus influenzae was
77.8% of the total strains of Haemophilus isolated.
Furthermore, the relationship between the isolation of the
organism, and the season, type of media, and age groups has been
detected. The organism has been isolated in Autumn and Winter
(one strain late Autumn, and eight strains in Winter), whereas no
isolation took place in Summer; (GC חagar) was found to be the
best medium for isolation compared to Chochalate agar. Children (3-
18year) and elderly(67-73year) were the most affected groups.
The study proved that the organism can be preserved in (4-8) °C
for about (10-15) days.
