Abstract:
This is a retrospective case control study aimed to study
the role of Toombak on deficiency of P53 among oral squamous
cell carcinoma patients.
The study was conducted in the Oral Pathology
Department, Faculty of Dentistry, University of Khartoum,
during the period from December 2009 to October 2010.
Sixty six formalin fixed, Paraffin wax embedded tissue
blocks were selected from tissues of patients with Oral
Squamous Cell Carcinomas (OSCC) 24 patients were Toombak
dippers and 42 patients were non dippers. Tissue sections were
stained using EPOS immunohistochemical method for detection
of P53 marker. The obtained data were analyzed using SPSS
computer program.
A total of 66 patients with oral squamous cell carcinoma
were investigated in this study, their ages ranged between 23
to 95 years with mean age of 59 years old
Out of 66 patients with OSCCs, 39 (59.1%) patients were
males and 27(40.9%) patients were females.
P53 was
expressed in 19 (49%) of males and 15 (56%) of females.
Out of 34 patients, positive expression was demonstrated
in 20 (58.8%) well differentiated OSCCs, 7 (20.6%) moderately
differentiated OSCCs, and 7 (20.6%) poorly differentiated
OSCCs, consequently of 32 negative expression, 19 (59.4%)
were well differentiated OSCCs, 11 (34.4%) were moderately
differentiated OSCCs, and 2 (6.2%) poorly differentiated OSCCs.
Out of 34 patients, positive expression was demonstrated
in 12 (35.3%) Toombak dipper patients and 22 (64.7%) non
Toombak dippers, consequently of the 32 P53 negative
expression, 12 (37.5%) were Toombak dippers and 20 (62.5%)
were non Toombak dippers.
These results show that P53 over expression is not affected
by Toombak use or by patient sex, age, and histological
grading of the tumor among Sudanese OSCCs patients.
