Abstract:
This study was carried out in the period from May 2007- March 2008 to
detect the presence of Salmonella typhimurium in stool of patients who were
suffering from acute gastrointestinal tract infection. Forty seven stool
specimens were collected from patients who were attended to Bashair
Hospital, Omdrman Teaching Hospital and Haj Alsafi Hospital during the
study period.
Bacterial DNA was extracted from each stool specimen.The DNA was
runned using real time PCR technique to detect S .typhimurium
The result revealed that only two specimens were positive.
The study concluded that the real time PCR technique facilitates detection of
S .typhimurium without bacteriological culture.
