Establishment of Normal Reference Values of White Blood Cells and Differential Count among Sudanese healthy Adult Population in Khartoum State

Abstract

This study was descriptive cross sectional survey to establish reference values on the white blood cells and differential white blood cells count in adult healthy Sudanese in Khartoum State, and compare them to previous study and international reference values. It also aims finding the effect of gender and age on the white blood cells and differential white blood cells count. This study included 500 healthy adult Sudanese populations from both sex in the age group (15-60) years, all of them from Khartoum state, they were interviewed according to specially designed questionnaire form and venous blood samples were collected from each after verbal consent. The blood collected in K3EDTA container, the samples were analyzed in the laboratory of Sudan judiciary clinic in Khartoum State by using automated hematology analyzer (Sysmex KX-21), and preparing ideal thin blood film to count differential white blood cells after staining by using leishman stain to give the percentage of differential white blood cells, then calculate the absolute value, the result analyzed by using computerize statistical package for social science (spss) (version11.5). The reference values for selected hematological parameters (white blood cells and differential white blood cells count) in study group in comparing with previous study- the white blood cells count show same result but when compare with international reference values, but differential white blood cells count In comparing with international reference values there is no significant difference (P.value > 0.05) in exception of lymphocyte with slightly increase in relative count and in absolute count. The reference values for white blood cells count (mean ± SD) 5.63 ±1.55×109/L the reference value is (4.06 -7.18×109/L), The reference value for differential white blood cells count (mean ± SD), Neutrophil 48.8% ± 8.9 the reference value is (39.9 % - 57.7 %), Lymphocyte 42.3% ± 9 the reference value is (33.3% - 51.3%), Monocyte 5.8% ± 1.9 the reference value is ( 3.9 % -7.7%), Eosinophil 2.2 ± 1.1 the reference value is (1.1% - 3.3%) and Basophil 00%, among gender as follow: Neutrophil: male 49.45 ± 8.9 %-female 50.26 ± 9 %, Lymphocyte: male 42.66 ± 9% -female 41.78 ± 8.9 %, Monocyte: - male 5.78 ± 1.9% -female 5.78 % ± 1.9, Eosinophil: male 2.10 ± 1.1%-female 1.2 ± 2.4%, and Basophil 00%. In study group gender showed different between male and female in TWBCs, but not significant. Differential white blood cells count among study group, show that all parameter within normal range except lymphocyte there is an increase number when compare with the normal value, and show absent of basophil in this study. There is a real need for a large sample from different Sudan States to evaluate the exact normal reference value of hematological parameter among Sudanese people in different age group.