Abstract:
Staphelococcus aureus. Pseudomonas and Proteus species
are most common cause of hospital acquired or nosocomial
infection, especially in patients with post-surgical wound,
diabetic and immunocompromised.
This study was conducted in the Royal Medical Laboratory in
Wad Medani during July to September 2013. It was aimed to
investigate the antibacterial activity of garlic extract against
three bacterial species (S. aureus. Pseudomonas aeroginosa
and Proteus species). Thirty five wound swab samples were
collected from patients with post-surgical and diabetic
wounds who were hospitalized in Wad- Medani Teaching
Hospital. The 35 samples were collected and cultured in the
laboratory, 8 S. aureus, 7 Pseudomonas and 5 were Proteus
species were isolated from the collected samples . Sensitivity
tests
were
done
to
the
20
isolates
by
using
two
concentrations of fresh garlic extract (1 g/ml, 500 mg/ml), on
Muller Hinton agar medium. The results showed that; at the
concentration of 1 g/ml, the mean diameter of zone was 30.8
for S. aureus, 28.5 for Pseudomonas species, 23 for Proteus
species
and
0
for
pseudomonas
aeroginosa.
At
the
concentration 500 mg/ ml the mean diameter of zone was
24.8, 24.5, 19 and 0 respectively. Overall results of this study
indicate that Allium sativum have an activity that can
prevent the growth of S. aureus, Pseudomonas and Proteus
species
on
Muller
concentrations.
Hinton
agar
(in-vitro)
at
different
