Detection of MUM1 Protein receptors among Sudanese Lymphoma’s Patients

Abstract

This is retrospective descriptive study performed in Khartoum state, during period from March to July 2012. The study aimed to identify the expression MUM1 protein receptor among Sudanese with lymphoma’s patients.. Thirty blocks were collected from patients with lymphoma attending to Radio Isotope Center in Khartoum by using simple random selection method. Samples were collected by surgical method then processed and sectioned using the standard techniques. Sections were stained using two method, histochemical methods using haematoxylin and eosin for histopathology diagnosis and immunohistochemical methods using polymer base technique for detection of MUM1 protein receptors. In addition the results of CD30, 15, and CD20 were collected from file. The ages of the involved patients ranged between one year to75 years with mean age 28 (±23.3) years old. Most of the patients were aggregating at age ranges older than 41 years representing 18 (60%) and the remaining 12 (40%) were younger than 41 years. In this study 20 (66.7%) of the involved patients were males and 10 (33.3%) patients were females. Out of 30 patients, histopathological diagnosis revealed diffuse large B-cell non Hodgkin’s lymphoma’s (NHL) in 18 (60%) patients, 5 (16.7) patients represented as mantle NHL and the remaining 7 (23.3%) patients were classical Hodgkin’s lymphoma’s (HL). In this study MUM1 receptor status showed positive expression in 15 (50%) patients, and negative in 15 (50%) patients. Among the study subjects CD30 and CD15 receptors were positive in 7 (23.3%) patients, and negative in 23 (76.7%) patients. CD20 receptor status was positive in 23 (76.7%) patients, and negative in 7 (23.3%) patients. Among the study subjects 18 (60%) patients of the diagnosed samples with diffuse large B cell NHL, 10 (55.6%) patients were males and 8 (44.4%) patients were females. Mantle cell NHL was represented by 5 (16.7%) patients, 4 (80%) patients were male and 1 (20%) was female. 7 (23.3%) of the selected samples were classical HL, 6 (85.7%) were males and 1 (14.3%) was female with statistical significant association (P. value < 0.05). Histopathological diagnosis and MUM1 receptor expression showed that positive in 10 (66.7%) patients and negative in 8 (53.3%) patients among diffuse large B-cell NHL. 5 (33.3%) positive patients and 2 (13.3%) negative patients were classical HL. 5 (33.3%) has no reaction with MUM1 protein represented as mantle cell NHL with statistical insignificant association (P. value > 0.05). Among the study subjects, CD30 status showed positive expression in 7 (23.3%) patients and negative in 23 (76.6%) patients, among the 7 (23.3%) patients positive for CD30, MUM1 show positive expression in 5 (33.3%) patients with statistical insignificant association (P. value > 0.05). Among the study subjects, CD15 status showed positive expression in 7 (23.3%) patients and negative in 23 (76.6%) patients, among the 7 (23.3%) patients positive for CD15, MUM1 show positive expression in 5 (33.3%) patients with statistical insignificant association (P. value > 0.05). Among the study subjects, CD20 status showed positive expression in 23(76.6%) patients and negative in 7 (23.3%) patients, among the 23 (76.6%) patients positive for CD20, MUM1 show positive expression in 10 (66.7%) patients with statistical insignificant association (P. value > 0.05). The histopathological diagnosis and CD30, CD15 and CD20 receptors status showed that 7 (23.3%) of demonstrated samples were positively expressed CD30 and CD15 marker, were CHL. 23 (76.7%) patients were CD20 positive were diffuse large B cell NHL and mantel cell NHL with statistical significant association (P. value < 0.05) In this study we conclude that MUM1 receptor expression among Sudanese patients with lymphoma is associated with lymphomas type.