Abstract:
This is a descriptive prospective hospital base study, was
conducted in Damazine Military hospital - Blue Nile state, during
the period from March to July 2012. Aimed to detect CMV in
product
of
conception
(aborted
tissue)
using
histochemical,serology and immunohistochemical methods.
Fifty samples were collected from females suffering of miscarriage
for one time or recurrent attending Damazine military hospital,
their ages were ranged between16 to 41 year, with mean age 28.5
years.
5o sample (biopsy and serum) ,biopsies were fixed in
formalin,prossed in paraffin and cut using routary microtom, two
section were prepared from each block. One section was stained
using Avidin-Biotin technique for immunohistochemical method to
detect CMV antibodies, another section was stained using
Heamatoxylene and Eosin for histological method to detect CMV
inclusion bodies, serum samples were used for CMV (IgG) using
ELISA commercial diagnostic kits (DRG Instruments GmbH.
Germany), data were analyzed using SPSS computer program.
Immunohistochemestry result releave 10(20%) sections were
positive, while 40(80%) were negative.
Histopathological
results
showed
23(46%).While 27(54%) no changes.
IV
changes
of
CMV
in
CMV IgG antibodies were present in the serum samples of all
women tested with ELISA.
This study showed insignificant relation between age, ethnic
origin, economic status, education level, occupation, number of
parity, blood transfusion, Intra Uterine Fetal Death (IUFD).
Congenital malformation and Immunohistochemical result.
The study concluded that The CMV IgG results indicate high
frequency of past HCMV infection in all tested groups;
Histopathology still represents accurate results of CMV infection.
