Abstract:
In this study, the stability of indomethacin was investigated in
different physical and chemical conditions using various analytical
techniques. Ultra-violet spectrophotometric studies of the drug in several
solvents and at a wide range of pHs were carried out. Isobestic points in
the UV spectra were located. Both solid and liquid indomethacin were
exposed to direct sun rays. Increasing the temperature as high as 120 °C.
Applying various analytical techniques, HPLC was found to be the most
sensitive to detect the decomposition of the drug.
Indomethacin was subjected to alkaline hydrolysis that resulted in
the production of two different compounds. These compounds were
isolated and identified by various analytical techniques, such as TLC,
GC/MS, UV, and IR spectrophotometric methods. The identification was
supported by the study of the kinetic behaviour and the derivatization of
the analyte drug. The products of the alkaline hydrolysis were identified
as p-chlorobenzoic acid and 2-methyl-5-methoxy-indol-3-ylacetic acid.
