Abstract:
The aim of this study was to detect Gardnerella vaginalis,
the main causative agent of bacterial vaginosis, more than
other opportunistic anaerobic organisms that colonized
the vagina.
Out of
150 women who visited the Obstetrics
and
Gynecology Clinic in Rabia Hospital, Riyadh- Saudi Arabia
during the period between July 2011 to June 2012, were
included in this study. 92/150 (61.3%) have vaginal
infection. Gardnerella vaginalis was n=74/92 (80.4%) was
isolated from
women with bacterial vaginosis, while
Candida species, Streptococcus species and Trichomomas
vaginalis were 16.3%, 2.2% and 1.1 % respectively.
Isolated Gardnerella vaginalis were examined for biotype
(hippurate hydrolysis, catalase and oxidase activity) and
genotype
with
molecular
technique
(PCR)
Amplified
ribosomal DNA (16S rDNA). This work concluded that
Gardnrella vaginalis is most bacterial vaginosis causative
agent in women of reproductive ages in Riyadh –Saudi
Arabia.
