Abstract:
The study was carried out under control laboratory conditions at
Faculty of Veterinary Medicine of Khartoum University during January
2005, to evaluate the effect of the Dwarf bee honey( Apis florea) and
chemical antibiotic Gentamicine on the three species of wounds bacteria
relates to Family: Enterobacteria.
Tow samples of honey were collected from wild nests from Khartoum
North area.(Elfakee Hashim and Elgiley sites). Chemical analysis of the
different components of the samples was carried out immediately at
Industrial Research and Consultancy Center (IRCC).The result confirmed
the previous studies. The Bacteria species brought from Sudan National
Health Laboratory. Honey concentrations were prepared by diluted
solution of honey in distilled water, and three concentrations of honey
were prepared (25%, 50% and 100%). Disk of filter paper equal in size to
antibiotic disks (Gentamicin10mg) were embedded in different
concentrations of honey for 24hours in 37°C. Disk sensitivity test was
chosen to conducted the study.
Experimental units were arranged in Completely Randomized
Black Design (CRBD) with three replicates.
Honey at 100% concentration after 24h incubated at 37°C gave
the highest mortality almost similar to chemical antibiotic
(Gentamicine10mg) on Escherichia coli and Proteus mirabilis but in
Klebsiella pneumonia the effect was equivalent to chemical antibiotic.
