Abstract:
This descriptive retrospective hospital based study.
conducted at Radiation and isotope center-Khartoum (RICK)
and Sudan university of science and technology college of
medical laboratory science during the period from May to
November 2013. The study was aimed to evaluate the
validity of high molecular weight cytokeratin (HMW) tumor
marker in the differential diagnosis of prostate tumors.
Thirty paraffin blocks previously diagnosed as prostate
tumors were taken randomly using simple random
method(twenty
adenocarcinoma
and
ten
benign
hyperplasia). Tissue sections were prepared and stained for
immunohistochemistry to detect HMWCK using new indirect
method (CELL MARQUE - clone 34βE12).
Patients data were collected from the patient’s files, the
patients age ranged between 52 – 88 years with mean age
67 years. The majority of patients were older than 60 year
(80%) and remainder ≤ 60 year (20%).
The result of HMWCK expression among study population in
20 adenocarcinoma showed positive result in only one case
(5%) and negative result in 19 (95%) , while in 10 benign
prostatic hyperplasia the expression of HMWCK were positive
in 9 (90%) and negative in only one case(10%), with
significant relation between HMWCK expression and tumor
behaviour.
The study concluded that HMWCK tumor markers can be
useful in the differential diagnosis of prostate tumors,
whereas positive expression associated with benign
hyperplasia and negative expression associated with
prostate adenocarcinoma.
