Abstract:
This is a hospital based descriptive retrospective study. It was conducted
at Souba Teaching Hospital, Omdurman Military Hospital and Sudan
University of Science and Technology (Khartoum state), during period
from April to August 2013, aimed to detect P53 and Bcl-2 expression in
colorectal tumors.
A total number of 50 tissue paraffin blocks previously diagnosed as
colorectal tumors were selected. 40 (80%) samples were colorectal
adenocarcinoma and 10 (20%) samples were colorectal adenomatous
polyps. Their age ranged from 5 to 78 years old with mean age of 49
years, 30 (60%) were males and 20 (40%) were females. Tissue section
were cut and stained using haematoxylin and eosin method to confirm
histopathological diagnosis and new indirect method for histochemical
detection of p53 and Bcl-2. The obtained data was analyzed using SPSS
program version 11.5. Frequencies mean and chi square test value was
calculated.
Colorectal adenocarcinoma showed 17 (42.5%) samples were well
differentiated adenocarcinoma, 11 (27.5%) samples were moderately
differentiated
adenocarcinoma,
while
6
(15%)
samples
poorly
differentiated adenocarcinoma, and the rest 6 (15%) samples were not
graded adenocarcinoma.
Positive p53 expression was detected in 23 (46%) samples of colorectal
adenocarcinoma with on expression in adenomatous polyps. 17 (32%)
and 10 (20%) samples of colorectal adenocarcinoma and adenomatous
polyps, respectively showed negative nuclear p53 expression.
Concerning bcl-2, positive expression was detected in 29 (58%) samples
of colorectal adenocarcinoma and only one (2%) sample of adenomatous
polyps, while 11 (22%) and 9 (18%) samples of colorectal
adenocarcinoma and adenomatous polyps, respectively revealed negative
bcl-2 expression.
This study found that there is significant relation between age group and
colorectal adenocarcinoma, also showed significant relation between
colorectal adenocarcinoma and p53 expression, as well bcl-2 expression
and colorectal adenocarcinoma, while there is no significant relation
between p53 and bcl-2 expressions in colorectal adenocarcinoma.
This study concluded that p53 and bcl-2 expression associated with
malignant colorectal tissue compared to benign tissue, as well as no
significant relation between p53 and bcl-2 expression and colorectal
adenocarcinoma grades.
