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Detection of Shigella flexneri in Stool Specimens using Real-time Polymerase Chain Reaction Technique
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| dc.contributor.author | Darmos, Khalid Abdalla Suleiman | |
| dc.contributor.author | Supervisor,- Humodi Ahmed Saeed | |
| dc.date.accessioned | 2014-02-10T07:21:16Z | |
| dc.date.available | 2014-02-10T07:21:16Z | |
| dc.date.issued | 2008-01-01 | |
| dc.identifier.citation | Darmos,Khalid Abdalla Suleiman.Detection of Shigella flexneri in Stool Specimens using Real-time Polymerase Chain Reaction Technique/Khalid Abdalla Suleiman darmos;Humodi Ahmed Saeed.-Khartoum:Sudan University of Science and Technology,college of Medical Laboratory Science,2008.-38p. : ill. ; 28cm.-M.Sc. | en_US |
| dc.identifier.uri | http://repository.sustech.edu/handle/123456789/3400 | |
| dc.description | Thesis | en_US |
| dc.description.abstract | This study was carried out in the period from May 2007- March 2008 to evaluate the real-time PCR technique for direct detection of Shigella flexneri in stools. Forty-six stool specimens were collected from patients attended to Khartoum state Hospitals. Bacterial DNA was extracted directly from each stool specimen using phenol choloroform technique. The DNA amplified by real-time PCR using specific Shigella flexneri primers. Shigella flexneri DNA detected in 12 (26.1%) of the extract. The study concluded that the real-time PCR technique facilitates rapid direct detection of Shigella flexneri in stool without any culture. | en_US |
| dc.description.sponsorship | Sudan University of Science and Technology | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Sudan University of Science and Technology | en_US |
| dc.subject | Shigella flexneri | en_US |
| dc.subject | Stool-Specimens-Polymerase | en_US |
| dc.title | Detection of Shigella flexneri in Stool Specimens using Real-time Polymerase Chain Reaction Technique | en_US |
| dc.type | Thesis | en_US |
