SUST Repository
Detection of Salmonella paratyphi C in Blood Specimens by Real Time Polymerase Chain Reaction Technique
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| dc.contributor.author | Ali, Ghada Ahmed Mohammed | |
| dc.contributor.author | Supervisor,- Humodi Ahmed Saeed | |
| dc.date.accessioned | 2014-02-06T07:07:25Z | |
| dc.date.available | 2014-02-06T07:07:25Z | |
| dc.date.issued | 2008-01-01 | |
| dc.identifier.citation | Ali,Ghada Ahmed Mohammed.Detection of Salmonella paratyphi C in Blood Specimens by Real Time Polymerase Chain Reaction Technique/Ghada Ahmed Mohammed Ali;Humodi Ahmed Saeed.-Khartoum:Sudan University of Science and Technology,college of Medical Laboratory Science,2008.-37p. : ill. ; 28cm.-M.Sc. | en_US |
| dc.identifier.uri | http://repository.sustech.edu/handle/123456789/3385 | |
| dc.description | Thesis | en_US |
| dc.description.abstract | A preliminary study was conducted during the period of March 2007 to March 2008 to detect S. paratyphi C directly in blood. Forty seven blood specimens were collected from patients suffering from enteric fever in Khartoum State. DNA of each specimen was extracted using phenol chloroform method. S. paratyphi C was detected by the aid of real time PCR using Quantica thermocycler. Only 1 (2.1%) of specimens was found positive and the rest 46 (97.9%) were negative. The study concluded that the real time PCR facilitates detection of S. paratyphi C without bacteriological culture and identification. | en_US |
| dc.description.sponsorship | Sudan University of Science and Technology | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Sudan University of Science and Technology | en_US |
| dc.subject | Salmonella paratyphi C | en_US |
| dc.subject | Blood-Specimens | en_US |
| dc.title | Detection of Salmonella paratyphi C in Blood Specimens by Real Time Polymerase Chain Reaction Technique | en_US |
| dc.type | Thesis | en_US |
