Abstract:
This study was carried out during the period from May 2007 to March 2008 to detect
the presence of Enterobacter cloacae in stool specimens from patients suffering from
gastrointestinal disturbance. Forty six samples were collected from patients attended
to Khartoum Teaching Hospital, Omdurman Teaching Hospital, Khartoum pediatrics
Hospital and Omdurman pediatrics Hospital.
DNA was extracted directly from stool specimens and used for the subsequent
experiment.
The forty six specimens were examined using Real time PCR Technique. The study
revealed only seven (15c/o) specimens were positive and the remained specimens
thirty nine (85%) were negative.
The study concluded that the real-time PCR technique facilitates rapid detection of
.bacterial pathogens directly from clinical specimen without culturing of bacteria
