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Detection of Salmonella enteritidis in Stool Specimens by Real-time Polymerase Chain Reaction Technique

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dc.contributor.author Mohammed, Abdelrahman Eldaw
dc.contributor.author Supervisor,- Humodi Ahmed Saeed
dc.date.accessioned 2014-02-05T11:16:02Z
dc.date.available 2014-02-05T11:16:02Z
dc.date.issued 2008-01-01
dc.identifier.citation Mohammed,Abdelrahman Eldaw.Detection of Salmonella enteritidis in Stool Specimens by Real-time Polymerase Chain Reaction Technique/Abdelrahman Eldaw Mohammed;Humodi Ahmed Saeed.-Khartoum:Sudan University of Science and Technology,college of Medical Laboratory Science,2008.-37p. : ill. ; 28cm.-M.Sc. en_US
dc.identifier.uri http://repository.sustech.edu/handle/123456789/3379
dc.description Thesis en_US
dc.description.abstract This study was carried out in the period from May 2007- March 2008 to detect the presence of Salmonella enteritidis in patients suffering from gastroenteritis. Forty-seven stool specimens were collected from patients attending Albangadid teaching hospital and Alzahra medical centre. Bacterial DNA was extracted from each stool specimen using phenol choloroform technique. Real-time PCR technique was adopted to detect presence of Salmonella enteritidis. The result revealed that only one (2.1%) specimen was positive. The study concluded that the Real-time PCR technique facilitates detection of bacterial pathogens without bacteriological culture. en_US
dc.description.sponsorship Sudan University of Science and Technology en_US
dc.language.iso en en_US
dc.publisher Sudan University of Science and Technology en_US
dc.subject Salmonella enteritidis en_US
dc.subject gastroenteritis en_US
dc.title Detection of Salmonella enteritidis in Stool Specimens by Real-time Polymerase Chain Reaction Technique en_US
dc.title.alternative ‫كشف السلمونيلة ملهبة الامعاء في عينات البراز بتقنية‬ ‫تفاعل البلمرة المتسلسل الزمني‬ en_US
dc.type Thesis en_US


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