Genetic Variant of Ribosomal protein S20 Gene among Sudanese Patients Diagnosed with Myocardial Infarction

Abstract

Arterial thrombosis and venous thrombosis are major causes of morbidity and mortality in the world and myocardial infarction is the common cause of disability and death in the world, cause of 10-25%of death in Sudan and its occupied 75% from cardiovascular disease in sub Saharan in Africa. Ribosomal proteins disorder associated with different disease like cancers and cardiovascular diseases. Ribosomal protein S20gen located in chromosome 8 P13.1 in the second intron which belong to 40 S subunit to S10P family and act as activator to protein 53 so it may have acritical role in coagulation modulation and any abnormality of this gen may affect the role of cardio and endothelial cell which response to protein 53 expression. ribosome protein S20 (A88T) polymorphism was detected in Diamond black fan anemia. And this study aimed to detect ribosome protein S20 polymorphism in Acute myocardial infarctions.

this study was descriptive cross sectional hospital based conducted in Alshaab hospital in Khartoum state from March 2022 to January 2023 with 47 patient diagnosed with myocardial infraction by ECG and cardiac enzymes, Clinical data were collected by non-self-questionnaire and recording forms, two ml of venous blood was collected from each patient with myocardial infarction in EDTA tube. And DNA was extracted by ammonium chloride method (salting out method). blood was genotyped by PCR enzymes fragmentation method and result was analyzed by SPSS version 26 program to calculate mean, standard deviation, for correlation T test and Chi -square.

Mean age of myocardial infarction patient is 58 ± 10 and 76% most of them were males, the majority of patient came from Khartoum State, the common clinical symptoms were chest pain 86.2% and about 44% of patient had a pervious history of myocardial infarction. And in chronic disease the hypertension represents 31% and diabetes mellitus 27% both 21%, risk factors under study included: obesity29% and smoking42%. all study population had a wild type of allele AA so all study population is negative for RPS20 polymorphism, and significant increase in total white blood cell in MI patient whom had a chronic disease that ribosomal protein S 20 A88T polymorphism is not associated with myocardial infarction.