Bacteriological and Clinical Study on Diarrheic Sheep Salmonellosis in Khartoum State, Sudan

Abstract

The aim of this Study was to estimate main clinical parameters of sheep suspected to have salmonellosis and investigation presence of Salmonella causing the disease and isolation and identification of bacteria causing the disease. A total of 53 sheep with abdominal pain and fluid diarrhea were examined clinically for pulse rate, respiration and body temperature in February to December 2020 in Omdurman locality, Khartoum State. Fecal samples were collected from these animals for bacteriological examination. MacConkey and salmonellashigella agar media were used for culturing and Gram's stain was used for examining the morphology of bacteria. Biochemical testswere applied for identification of the bacteria. The results, revealed that the pulse rale was (110.00±1.01 to 99.10±1.00), respiration (38.10±2.10 to 35.10±1.10) and body temperature (40.21±1.00 to 39.10±0.20) were increase the culture of the fecal samples in MacConkey agar medium showed that 19 (35.8%) the colonies were small, round, slightly elevated glister of yellow color that did not ferment of lactose and glistening colonies, while in salmonella shigella agar medium revealed black colonies. The results of Gram's stain showed gram-negative rod-shaped bacteria in culture of fecal sample. Biochemical tests showed that all the isolates were positive to catalase test and negative to oxidase, indole and urease test. In conclusion, the disease is prevalent in the study area in sheep which leading to economic loss due to death of the animals, costs of laboratory diagnosis, treatment of clinical cases, cleaning and disinfection and also control and prevention costs.