Detection of 23SrRNA Gene Mutations Related to Clarithromycin Resistance of Helicobacter pylori Isolated from Dyspeptic patients in Khartoum state

Abstract

Helicobacter pylori infection is a major gastric infection and clarithromycin resistance is increasing worldwide, data regarding resistance are limited in developing countries. This cross-sectional study was conducted in Khartoum-Sudan in the period from June 2018 until April 2019 to detect the clarithromycin resistance mutations in the 23SrRNA genes isolated from Helicobacter pylori among dyspeptic patients in Khartoum state. A seventy-one gastric biopsies were collected from patients undergoing gastroduodenal endoscopy, DNA using the guanidine method then polymerase chain reaction to detect 16SrRNA and 23SrRNA genes Then all the positive results were subjected to sanger sequencing to detect mutations associated with clarithromycin resistance. Out of seventy-one specimens, forty-two patients (59.2%) were positive for 16SrRNA gene from which nineteen specimens (26.8%) were positive for the 23srRNA gene. the prevalence of mutations that is related to clarithromycin resistance was (7.1%) with only two Samples had the A2143G mutation (2.8%), two samples had the T2182C mutation and one Sample had both A2143G and T2182C mutations, but there is no significant correlation between the presence of these mutations and age, gender or disease. This study concluded that Clarithromycin resistance of Helicobacter pylori in this study is low but there are mutations that can affect the eradication therapy.