Detection of Plasmid Mediated AmpC β-Lactamase Genes in Enterobacteriaceae Isolates from Hospitalized Patients in Khartoum State

Abstract

Plasmid-mediated class C β-lactamases are reported from Enterobacteriaceae with increasing frequency. They likely originate from chromosomal AmpC of certain Gram-negative bacterial species and subsequently are mobilized onto transmissible plasmids. The objective of this study was to detect plasmid mediated AmpC β-lactamase among Enterobacteriaceae isolates from hospitalized patients in Khartoum State, Sudan using phenotypic test and multiplex PCR Assay. A total of 76 clinical isolates of Enterobacteriaceae were included in this descriptive-cross sectional laboratory based study. The isolates were collected from hospitalized patients in Khartoum State, including both males and females with different age using non-self-constructing information form. The isolates were stored in 20% glycerol peptone medium and inculated in MacConkey agar. Antbiotic susceptibitity test was carried out using Modified Kirby Bauer technique. Confirmation of the AmpC β-lactamase production was done by Cefoxitin-cloxacillin double disk synergy test. DNA was extracted using guanidine chloride method and AmpC β-lactamase genes was detected by used multiplex PCR assay. Out of 76 isolates; 9( 22.0%) isolates were found positives by Cefoxitin-cloxacillin double disk synergy test. Plasmid mediated AmpC β-actamase genes were detected only among 6(7.9%) of the total isolates. Detection of Amp C production is crucial in order to establish the antibiotic therapy and to attain the favorable clinical outcomes.