Phenotypic Detection of AmpC enzyme in Gram - negative Bacteria Isolated from Patients with Urinary Tract Infection in El Obaied Teaching Hospital

Abstract

This study was done to detect the presence of AmpC enzyme among Gram negative bacteria isolated from patients of urinary tract infection attending EL-obaied Teaching Hospital. The study was conducted during the period from Jun 2018 to February 2022. A total of 100 urine specimens were collected from patients suspected with urinary tract infection. These were 13 from males and 87 from females. The specimens were cultured on CLED agar for primary isolation. The identification of Gram-negative bacteria was done by Colonial morphology, Gram stain and biochemical tests. Antimicrobial sensitivity tests were carried out by Kirby-Bauer Disk Diffusion Method. Detection of AmpC enzyme was done by non-extract based modified three dimension test. The result revealed that 52 Gram-negative bacteria were isolated. The isolates were 15 Escherichia coli, 9 Enterobacter aerogenes, 9 Proteus mirabilis, 8 Kelbsiella pneumoniae, 4 Pseudomonas aeruginosa, 3 Citrobacter freundii, and one Proteus vulgaris. AmpC enzyme was detected in 24(46.2%) out of the 52 isolates. The distribution of AmpC as follows; 4(16.7%) Escherichia coli, 7(29.1%) Enterobacter aerogenes, 1(4.2%) Proteus mirabilis, 2(8.3%) Klebsiella pneumonia, 4(16.7%) pseudomonas aeruginosa, 2(8.3%) Citrobacter freundii, 3(12.5%) Serratia marecesns and the 1(4.2%)Proteus vulgaris. The study concluded that there is a high ratio of bacteria that are AmpC enzyme producers in UTIs patients in EL-obaied Teaching Hospital.