Effect of Blood Storage at Temperature (4C°) on Complete Blood Count Parameters

Abstract

Background: Complete blood counts are done to monitor overall health, to screen for some diseases, to confirm a diagnosis of some medical conditions, to monitor a medical condition, and to monitor changes in the body caused by medical treatments. Most blood counts today include a WBC Count and leukocyte differential count (LDC) (that is, not just the total WBC count but also the count of each WBC type, such as neutrophils, eosinophils, basophils, monocytes, and lymphocytes). More sophisticated modern analyzers can provide extended differential counts. Objectives: This study aimed to assess the effect of Storage at Temperature (4C0) on Complete Blood Count Parameters. Materials and Methods: This is cross sectional study conducted in Khartoum state during the period from March 2019 to January 2021. It included 70 samples, complete blood count conducted to all 70 samples freshly(base line) and after 24hours storage at Temperature (4C0). Sysmex xp-300 hematological analyzer was used to estimate CBC. The statistical analysis was performed using SPSS version 21. Results: the results of study shows that the mean level of HB ,HCt, MCV,MCH, MPV, PDW and PLCR were significantly increased after storage for 24hrs compared to base line results (12.6 ± 2.1; 12.7 ±2.1, p value= 0.000), (39.08 ±0 .76; 39.4 ± 0.78, p value= 0.000), (84.7 ± 5.3; 84.9 ± 5.4, p value= 0.000), (27.4 ± 2.1; 27.5 ± 2.1, p vale= 0.001) , (9.5 ± 0.95; 10.7 ± 0.99, p value= 0.000),(11.7± 1.8, 13.8±2 ,P vaue= 0.000),(22± 6.9: 31.1± 7.5,p value0.000) respectively, while the mean level of RDW and Platelets were significantly decreased after storage for 24hrs compared to initial results (13.6 ± 1.7; 13.9 ± 1.7, p value= 0.000), (318.8 ± 119.9; 305.2 ± 118.4 , p value =0.000). In contrast, RBCs, MCHC,WBCs, Lymph %, Lymph Count, Neut %, Neut count, MXD%, MXD count and PCT show no difference between base line and 24hrs reading (4.57± 0.7: 4.58± 0.7,p value= 0.708),(32.27 ± 1.21: 32.25± 1.25,p value= 0.758),(7.22± 2.6: 7.09± 2.6,p value= 0.180),( (2.23 ± 0.76; 2.22 + 0.78, p value=0.703), (57.3 ± 12.5; 132.4 ± 639.1, p value = ±0.327), (4.3 ± 2.3; 4.1 ± 2.1, p value= 0.125), (10.23 ± 3.9; 11.5 ± 6, p value= 0.195), (0.702 ± 0.28; 0.705 ± 0.36, p value= 0.947), (0.30 ± 0.10; 0.59 ± 2.2, p value= 0.278) respectively. Conclusion: This study conclude that, the level of Hb, HCt, MCV, MCH, PDW and PLCR were increased , the level of RDW and Platelets were decreased while RBCs, MCH, WBCs and differential leukocytes were not affected.