Constituents, Antimicrobial and Antioxidant Activity of Oils from Selected Plant Species

Abstract

The oils from targeted plant spies were extracted by n-hexane. Gas chromatography - mass spectrometry has been used for the identification and quantification of the studied oils. The extracted oils have been screened for Antibacterial Activity against five standard human pathogens. Citrullus colocynthis L. seed oil was characterized by GC-MS Revealed the presence of (22) components. Major constituents are : 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (46.30 % ), Hexadecanoic acid, methyl ester (16.35% ) , 9-Octadecenoic acid (Z)-, methyl ester (15.58% ) Methyl stearate (14.34% ) . Pimpinella anisum L. seeds oil was characterized by GC-MS Revealed the presence of (24) components. Major constituents are Anethole (82.75%) , Butanoicacid 2-methyl-, 2- methoxy-4-(2- propenyl) phenyl ester (6.25 %). Melissa officinalis L. seeds oil was studied by GC-MS. The analysis revealed (26) constituents. Major constituents are: 9,12- Octadecadienoic acid (Z,Z)-, methyl ester (52.94% ) - Octadecenoicacid (Z)-, methyl ester (12.01% ) , Hexadecanoic acid, methyl ester (11.25% ) and Methyl stearate (5.19% ) . `Origanum majarana L. seeds oil showed the presence of (35) constituents dominated by: 9,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z) (40.78% ) 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (33.61% ) , Hexadecanoic acid, methyl ester (11.00 %) and Methyl stearate (5.72 % ) . Raphanus sativus L. seeds oil gave total of (25) components major components are: 13-docosenoic acid, methyl ester, (Z)-(28.02%),cis-13-eicosenoic acid, methyl ester(15.35%) , 9,12- octadecadienoic acid -(Z)-, methyl ester (12.75%) and hexadecanoic acid, methyl ester (8.63%). Lepidium sativum oil showed total of (25) constituents .Major constituents are: cis-13-eicosenoic acid methyl ester (16.48% ), 9,12-octadecadienoic acid (Z,Z)-, methyl ester(15.90 % ) , hexadecanoic acid methyl ester(13.11%) and 9,12,15-octadecatrienoic acid methyl ester(Z,Z,Z)-(10.16%). Citrullus colocynthis L. seed At a concentration of 100mg/ml Oil was inactivate against Bacillus subtilis, partially active against Staphylococcus aureus, good against Pseudomonas aeruginosa and partially active against Escherichia coli. The oil failed to give any Antibacterial Activity against Candida albicans. . Pimpinella anisum L. seeds oil at a concentration of 100mg/ml the oil showed activate against Bacillus subtilis, partially active against Staphylococcus aureus and Pseudomonas aeruginosa and very activate against Escherichia coli. The oil failed to give any Antibacterial Activity against Candida albicans. Melissa officinalis L. seeds oil exhibited good activate against. Staphylococcus aureus, Pseudomonas aeruginosa Candida albicansand Escherichia coli. The oil failed to give any Antibacterial Activity against Bacillus subtilis. Origanum majarana L. seeds oil showed partially activate against, Staphylococcus aureus and Pseudomonas aeruginosa, Candida albicans and Escherichia coli. The oil failed to give any Antibacterial Activity against Bacillus subtilis. In the DPPH assay Raphanus sativeus oil showed IC50: 68.65±1.23 close to that of the standard antioxidant: butylated hydroxyl toluene (BHT) – (IC50 61.11±1.78). In the superoxide anion scavenging assay , the oil sample gave : IC50 123.19±7.63 while the positive controls showed : BHT(IC50, 60.5±0.22) and Trolox (IC50 54.98±0.17).However , in the metal chelating assay the oil showed IC50: 132.53±0.80) . The antioxidant activity of Lepidium sativum oil was conducted. The oil showed a free radical scavenging capacity in the DPPH assay of: (IC50 97.36±0.9). In the metal chelating assay, the studied oil showed IC50: 118.25±0.31. In the superoxide anion scavenging assay, the oil sample showed ( IC50 11.72±0.17) .