The GC-MS Analysis and Antimicrobial Activity of Oils from Potential Medicinal Herbs

Abstract

This research was carried out to study the oils from six potential medicinal plants used in Sudanese system of medicine. The oils from the targeted plants have been studied by gas chromatography- mass spectrometry and then the antimicrobial potential has been evaluated. The GC-MS analysis of Haplophyllum tuberculatum seed oil showed 22 components the major constituents are: 9, 12-octadecadienoic acid methyl ester (49.60%); 9, 12, 15-octadecatrienoic acid methyl ester (17.91%); hexadecanoic acid methyl ester (11.70%) and methyl stereate (8.07%). Brassica juncea oil showed 15 components. Major constituents of the oil are: -13-Docosenic acid methyl ester(43.61%); 9, 12-Octadecadienoic acid methyl ester(17.50%) ; 9 ,12, 15-Octadectrienoic acid methyl ester(12.49%) and cis-13-eicosenoic acid methyl ester(7.83%). Momordica chantia oil gave 21 components dominated by: 9,12-octadecadienoic acid methyl ester (47.64%) ; hexadecanoic acid, methyl ester (17.06%) and 9,12,15-octadecatrienoic acid methyl ester(13.29%). Cordia myxa oil exhibited 17 components the major are: 9,12-octadecadienoic acid methyl ester (28.81%); hexadecanoic acid, methyl ester (23.24%);11-octadecenoic acidmethyl ester(16.89%) and 11-octadecenoic acidmethyl ester(16.89%). Thirty one components were v detected in Helianthus annuus oil with major constituents: 9,12-octadecadienoic acid methyl ester (44.67%) ;9-octadecenoic acid methyl ester(19.71%) and hexadecanoic acid , methyl ester (17.16 %). Securidaca longipedum oil showed 22 components dominated by: 9 12-octadecadienoic acid methyl ester(29.04%) ; hexadecanoic acid methyl ester(22.90%) ; methyl stearate(15.30%) and 9 12 15-Octadecatrienoic acid methyl ester(7.10%). The oils have been evaluated for their antimicrobial activity against five human pathogens(Bacillus subtilis, Escherchia coli, Staphylococcus aureus Pseudomonas aeruginosa, Candida albicans) using the cup plate agar diffusion method. The oils showed various antimicrobial responses against the test organisms