Isolation of Aspergillus flavus From Poultry Feed in Khartoum State

Abstract

Aspergillus flavus is an important fungal species which mayoccurs in foods and feed, producing a number of toxins including aflatoxins.There are several diseases that affect poultry and cause severe losses; among which is mycotoxicosis caused by contaminated poultry feed with Aflatoxin-producing by strains of Aspergillus-flavus; consequently the research problem concerned the isolationof Aspergillus flavus from poultry feed.Aflatoxin-producing strains of Aspergillus flavus and these toxins affected poultry, animal and human. Poultry farms are suitable environments for growth of fungi. Most poultry feeds are prone to fungal growth during different stages of the manufacturing process, production, transportation and storage. Poultry are source of meat and eggs for human consumption.This study was carried out in Khartoum State, from different localities for identification of Aspergillus-flavus from food poultry cakes (Ombaz) and detection of Aflatoxins. Identification of the Aspergillus-flavusbased on the morphological characteristics of the colonies and microscopic examinations.Thirty samples (Cake) were collectedfrom different places in Khartoum State.(Omdurman, Bahri and Sharg-elnile). All samples cultured in Sabouraud’s Dextrose Agar (SDA),plates put in incubator for seven days in temperature 25 – 30 C. After the fungus growth,it was sub cultured in potato dextrose agar(PDA, Aspergillus-flavus had green colour colonies, the old colony appeared as dark green