Serological diagnosis and molecular detection of Brucella species in Camels in West Kurdufan state, Sudan

Abstract

The one humped camels (Camelus dromedarius) possess an economic importance in the Sudan as well as, in several other countries all over the world .Brucellosis is a worldwide zoonotic disease that is recognized as a major cause of heavy economic losses to the livestock industry and poses serious human health hazard.The aim of this study was to detect brucella in the camels blood . A total of 500 blood samples (serum and whole blood) were collected from Alnehood, Elkhwai and Ghubaish localities in West Kurdufan state and tested by Rose Bengal plate test (RBPT), Serum Agglutination Test(SAT) and polymerase chain reaction (PCR). Ninteen samples (3.8%), seventeen samples (3.4%) were found seropositive by RBPT and (SAT) respectively however, none of the sample was found positive by PCR. Despite the molecular method used in this study was highly sensitive and specific none of the sample was found positive for Brucella by PCR whereas nineteen and seventeen samples were positive by using Rose Bengal and Serum Agglutination tests SAT test respectively. The obtained results suggest that control and eradication programs for Brucella spp. infection seem to be necessary in camels.