Antimicrobial Activity of Trigonella foenum-graecum (fenugreek) oil against β-lactamase producers Escherichiacoli, Klebsella pneumoniae,and Pseudomonas aeruginosa using Gas chromatograph/mass spectrometery and molecular docking

Abstract

This a experimental study was conducted during the period from November,2019 to March,2020, aimed to testing the antibacterial activity, of T.foenum-graecum (fenugreek) oil using GC-MS and molecular docking against β lactames producer Escherichia coli, Klebsiella pneumoniae,and Pseudomonas aeruginosa . A total of forty five bacterial isolates were collected from from Almoaleem Hospital . These bacterial isolates were re identified by using different biochemical tests. the 45 bacterial isolates, 15(33.3%) were Escherichia coli,15(33.3%) were Pseudomonas aeruginosa and 15(33.3%)were Klebsiella pneumoniae. And they were tested for antibiotics sensitivity by using disc diffusion method, the antibiotics used were, Ciprofloxacin, Ceftazidime, Cefatoxime and Imipinem. The results showed that Ceftazidime had higher resistant rates (86.6%). Then test activity of T.foenum-graecum (fenugreek) oil against bacterial isolates by using cup plate agar diffusion method. The results showed that T.foenum-graecum (fenugreek) oil have antibacterial activity against β lactames producer Escherichia coli, Klebsiella pneumoniae,and Pseudomonas aeruginosa and reference isolate. The results revealed that Escherichia coli was high susceptible organism to T.foenum-graecum (fenugreek) oil and Klebsiella pneumoniae was low susceptible organism to oil. Then T.foenum-graecum (fenugreek) oil was analyzed by Gas chromatography–mass spectrometry (GC/MS) to detect chemical component of T.foenum-graecum (fenugreek) oil. The result showed 7different components. Then bioinformatics tools was use to docking fenugreekine, linoleic acid, methyle ester and palmitic acid, methyle ester with CTX-m15 enzyme. The result showed that the fenugreekine is the best one. T.foenum-graecum (fenugreek) oil showed high antimicrobial activity against β lactames producer Escherichia coli, Klebsiella pneumoniae,and Pseudomonas aeruginosa and reference isolate.