Immunohistochemical Detection of Hepar-1 Marker in Liver Carcinomas among Sudanese

Abstract

This is a hospital based descriptive retrospective case study conducted during the period from July 2018 to September 2019 in Khartoum state , the study aimed to assess the immunohistochemical detection of hepar-1 in liver carcinomas among sudanese . A total of 40 formalin fixed paraffin blocks previously diagnosed as liver carcinomas, 20(50.0%) samples were hepatocellular carcinomas, 10(25.0%) samples were adenocarcinomas and 10(25.0%) samples were metastatic carcinomas were selected for this study. One section of three microns was cut from each block and stained by immunohistochemical method (avidin biotin technique) for hepar-1 detection. The data obtained was analyzed using SPSS computer program version 20. The patient ages ranged between 29-85 years with mean age 57 years, most of patients 28(70.0%) were above 50 years and the remaining 12(30.0%) were less than 50 years. The patient sex revealed that 33(82.5%) of patients were male and 7(17.5%) patients were female. Positive expression of hepar-1 among study samples were 19/40 samples, distributed as follows: in hepatocellular carcinomas 15/20 samples, adenocarcinomas 2/10 samples and in metastatic adenocarcinomas 2/10 samples, while negative expression of hepar-1 among study samples is 21/40 samples, distributed as follows: in hepatocellular carcinomas 5/20 samples, adenocarcinomas account 8/10 samples, metastatic adenocarcinomas account 8/10 samples with significant correlation between hepar-1expression and liver carcinomas (P. value 0.002). The study coclude that the expression of hepar-1 is highly expressed in hepatocellular carcinomas.