Effect of Ramadan Fasting on Interleukin-6 and Some Hematological Parameter in Rheumatoid Arthritis Patients

Abstract

This was analytical cross section study conducted out in Omdurman military hospital and Alrian laboratory in Khartoum state. During the period from May 2019 to December 2019, the study aimed to investigate the effect of intermittent fasting Ramadan (14-16) hours on IL-6 and some hematological parameter in rheumatoid arthritis patients, 60 rheumatoid arthritis patients were selected randomly, and divided into fasting rheumatoid arthritis patient (n=30) and non- fasting rheumatoid arthritis patient (n=30) as control. Three ml of venous blood samples were collected from each subject in EDTA container. Complete Blood Count (CBC) was performed for All blood samples using sysmex s310, the plasma separate by using centrifugations. IL-6 level was measured in plasma samples by using ELISA kit (human IL-6, biolegand's ELISA MAXTM) (Western Blotting technique). The data were analyzed using Statistical Package for Social Science (version18) using independent T-test. IL-6 level was significantly decrease in fasting rheumatoid arthritis (143.29±123.75) compared with non-fasting rheumatoid arthritis (256.27 ± 253.52), (P.value = 0. 0 23). Hematological parameters (WBC, RBC ,Hb and HCT) were not affected significantly by fasting (P.value 0.400, 0.917, 0.140 and 0.203) respectively. This is study concluded that: intermittent fasting is favorable behavior for rheumatoid arthritis patients indicated by reduction of inflammatory marker IL6