Detection of Salmonella in yoghurt and cheese in Khartoum state – Sudan

Abstract

The study was conducted to isolate and identify Salmonella spp and to determine Total bacterial count in 60 yoghurt and cheese during 2017 in Khartoum state .A total of 60 samples of local yoghurt and cheese chosen randomly from retail points (local shopes and super markets) in Khartoum state ( Bahri ,Khartoum, Omdurman localities ).For isolation of bacteria the samples were cultured and for identification of these bacteria the biochemical tests were used .Total bacterial count was also used .The results revealed that there was no statistical difference between 3 localities (P> 0.05) after using total bacterial count of cheese and yoghurt samples .High total bacterial count of cheese was recorded in Omdurman (P=0.8801),while in Yoghurt was P=0.9491 recorded in Bahri .The contamination of cheese higher in local markets (137.08+ 2.84) than super markets(132.08 + 2.56),whereas in Yoghurt were higher in super markets(644.07+ 631.07) than local markets (141.08 + 247.07). The bacteria isolated from these samples were E.coli , Salmonella spp, Klebsiella spp and Proteus spp .In conclusion ,the contamination of milk by microorganisms due to handling ,equipment and machines which are normal flora, but can cause of the disease in non hygienic environment .