Evaluation of Serological Tests Used for Diagnosis of brucellosis in Goats intended for exportation in Sudan

Abstract

A total of 1500 sera samples were collected from Goats from Suakin Veterinary Quarantine for exportation. All sera samples were screened for antibodies against Brucella by Rose Bengal Plate Test (RBPT) as screening test the test was done in quarantine lab in Suakin. All positive sera which were 180/1500 (12%) were further retested by three tests and the passivity was as follows Buffered Acidified Plate Antigen (BAPA) 135/180 (75%), Serum Agglutination Test (SAT) 36/180 (20%) and Indirect Enzyme Linked Immunosorbent Assay (I-ELISA) 46/180 (25.6%). The statistical analysis-using kappa showed good relationship between RBPT, BABA, SAT and I- ELIS