The impact of hematological parameters, Reticulocyte Parameters and Blood Cell Morphology on Severity of disease Among Sudanese children with Sickle Cell Disease 2018

Abstract

This study is analytical Cross-sectional descriptive study conducted to estimate the impact of hematological parameters , reticulocyte Parameters and blood cell morphology on disease severity among hundred sickle cell Sudanese Children , in age group from (1-6) years in Khartoum state, at Jaafer Ibn Aouf Pediatric Hospital from January to November 2018 , a designed questionnaire was used for data collection accompanied with written informed consent for all participant , (2.5 ml) EDTA venous blood was collected under standard phlebotomy procedure, the complete blood count (CBC) was measured by using automated hematological analyzer (Sysmex xp-300) , reticulocyte parameters were calculated and blood films were done for each sample, sample analysis was done in Turkish medical diagnostic center and then data analyzed using statistical package of social science program version 19 , the results showed that the red blood cells count was ( 2.5 ± 0.5) × /l, hemoglobin (7.4 ± 1.2) g/dl, hematocrit (21.9± 3.9)%, red cell distribution width (59.9± 15.2 ) fl , mean cell volume (88.9 ± 11.9) fl , mean cell hemoglobin (30.1 ± 4.1) Pg, mean cell hemoglobin concentration (34.0 ± 2.4)g/dl, white blood cells count (15.3 ±6.6)× /L, Neutrophils (47.7± 11.6)%, Lymphocytes (42.0 ± 11.3) % , MID% (10.3±2.4) , platelets count (387.0±162.7) × /L, retics (5.4±3.1) %, corrected reticulocyte count (2.5± 1.4) % and reticulocyte production index (0.62±0.39) , peripheral blood picture show variety of cells with diagnostic value, hematological parameters showed significant correlation with anemia severity include red blood cells count (p.value =.000) , hemoglobin concentration (p.value=0.000) , hematocrit (p.value=0.000) , red cell distribution width (p.value=0.000) and white blood cells count (p.value=0.003), while other hematological parameters including mean cell volume , mean cell hemoglobin , mean cell hemoglobin concentration and platelets count showed insignificant correlation with anemia severity (p.value > 0.05) , retics count show significant correlation with anemia severity (p.value=0.005) , while corrected retics count and reticulocyte production index showed insignificant correlation with anemia severity (p.value > 0.05).