Antibacterial Activity of Garlic Allium sativum Extract Against Bacteria Isolated From Patients with Urinary Tract Infection in Khartoum State

Abstract

This was a cross sectional study conducted during the period from April to December 2019, aimed to testing the antibacterial activity of of Allium sativum ethanolic extract against urinary tract bacterial isolates. A total of one hundred bacterial isolates were collected from from Ribat University Hospital, Albaraha Hospital and Khartoum Army Hospital. These bacterial isolates were inoculated into nutrient agar slope for preservation and then re identified by using different biochemical tests. About 100 bacterial isolates, 35(35%) was Escherichia coli, then 25(25%) Pseudomonas aeruginosa followed by 20(20%) Klebsiella pneumonia, 11(11%) Enterococcus feacalis and finally 9(9%) proteus mirabilis. After re identification, these isolates were subjected to antibiotics sensitivity by using disc diffusion method, the antibiotics used includes Gentamicin, Ciprofloxacin, Ceftazidime, Cotrimoxazole and Imipinem. The results showed that Ceftazidime (67%) had higher resistant rates. Then test activity of different concentration (100, 50, 25, and 12.5) of Allium sativum ethanolic extract against bacterial isolates by using cup plate agar diffusion method. The results showed that Allium sativum have antibacterial activity against all pathogenic and reference strains. The results revealed that Enterococccus faecalis was more susceptible organism to Allium sativum extract and Pseudomonas aeruginosa was less susceptible organism to extract with mean of inhibition zones (20.8mm, 17.2mm) respectively at 100 (%w/v). Also the result showed that MIC of Garlic extract for all tested bacteria was 12.5 (%w/v).