Determination of Natural and Mineral Ingredients in Sudanese hair Cosmetic Products

Abstract

This study focused on the extraction of active natural products, to be used as hair cosmetics from some local herbal products, determination of heavy metals ,antioxidants and proteins in some natural herbal plants and animal fats used as hair cosmetic in Sudan. In the current study, 12 samples of Sudanese hair cosmetic products (3 samples were plant extracts and 9 were animal fats samples) were analysed.

Preliminary phytochemical screeninggave positive results for tannins and flavonoids, which are applied for hair as dye to give excellent color strength and smooth hair surface morphology, Active constituents of Sudanese medicinal plants were successfully extracted using 80% methanol. Hibiscus gave higher content of total tannins (189. 05 mg/dm3) than that given by colves (48.03mg/dm3). The latter, however, colves gave higher content of flavonoids (311.86mg/dm3)than that given by hibiscus(54.86 mg/dm3).Vitamin C was determined spectrophotometricly. The highest content of vitamin c was found in colves (1.155% w/v) ,the lowest in ginger (0.349% w/v ) and the middle in hibiscus (0.855% w/v) . A very simple HPLC method was performed to estimate niacine, gallic acid and ascorbic acid in ginger, hibiscus and colves samples using aqueous extract. Although ginger gave higher concentration of niacine (11.48 ppm), than that of hibiscus (1.04 ppm) and Colves (0.525ppm) and ginger gave higher concentration of gallic acid (56 ppm), than that of hibiscus (12.05 ppm) and colves (2.265 ppm). However, HPLC chromatograms of herbal samples did not show vitamin C peaks, because the method might not be suitable for its extraction. A simple, specific, economic, precise and rapid method for spectrophotometric determination of niacin in ginger was developed and validated. The ICP study measured higher concentration levels of zinc,(from 4.970 ppm to 34.24 ppm ), magnesium (from 2813 ppm to 4185 ppm ), silicon (from 43.25 ppm to 95.87 ppm ) and iron (from 196.21 ppm to 437.91 ppm )in plant extracts samples higher than those of animal fats samples (from 0.000112 ppm to 32.43 ppm ); The selenium concentration found to be the same in all samples (0.004993 ppm). The result of antioxidant for colves, ginger and hibiscus showed high content of phenolic compounds and polyphenols, they gave a strong antioxidant activities. Consequently, they showed a radical scavenging effect of 90.4% ,85.7%, 20.5%, respectively, in their ethanolic extracts.The effect, on Sesame Oil (48.1%),beeswax (26.4%), camel hump fats (14.3%), lamb grease (4%) and bone marrow fats (3.1%), was alsodetermined.