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Molecular detection of mecA gene in methicillin resistant Staphylococcus aureus isolated from food samples in Khartoum State

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dc.contributor.author Ahmed, Hashim Abdalbagi Ali
dc.contributor.author Supervisor, - Yousif Fadlallah Hamedelnil
dc.date.accessioned 2020-11-17T07:43:14Z
dc.date.available 2020-11-17T07:43:14Z
dc.date.issued 2019-01-01
dc.identifier.citation Ahmed, Hashim Abdalbagi Ali.Molecular detection of mecA gene in methicillin resistant Staphylococcus aureus isolated from food samples in Khartoum State\Hashim Abdalbagi Ali Ahmed;Yousif Fadlallah Hamedelnil.-Khartoum:Sudan University of Science & Technology,College of Medical Laboratory Science,2019.-57p.:ill.;28cm.-M.Sc. en_US
dc.identifier.uri http://repository.sustech.edu/handle/123456789/25417
dc.description Thesis en_US
dc.description.abstract This study was conducted in Khartoum State from August to November 2018. This study aimed to detect Staphylococcus aureus resistance genes isolated from food samples obtained from different retailers in Khartoum State, by conventional PCR assay. A total of four hundred samples were collected in this study. 84 (21%) of 400 of isolates were identified as S. aureus, 110 (27.5%) of 400 were identified as other than S. aureus and 206 (51.5%) of 400 yielded no growth. Oxacillin sensitivity test showed 31(36.9%) of 84 were Oxacillin resistant and 53 (63.1%) of 84 were Oxacillin sensitive. Finally PCR was done for the detection of resistace gene mecA in Staphylococcus aureus. The result of PCR assay for mecA gene was 15 of 84 (17%) positive and 69 (83%) negative. The distribution of mecA in types of samples was 17% in milk, 15% in fish, 20% in meat and 20% in cheese. furt en_US
dc.description.sponsorship Sudan University of Science & Technology en_US
dc.language.iso en en_US
dc.publisher Sudan University of Science & Technology en_US
dc.subject mecA gene en_US
dc.subject Staphylococcus aureus en_US
dc.subject methicillin resistant en_US
dc.title Molecular detection of mecA gene in methicillin resistant Staphylococcus aureus isolated from food samples in Khartoum State en_US
dc.type Thesis en_US


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