Antimicrobial activity of Aloe Barbadensis millar (Aloe Vera) against Pseudomonas aeruginosa isolates in Khartoum state, Sudan

Abstract

Aloe vera is medicinal plant with antimicrobial properties against bacteria, viruses and fungi. This study was done in Khartoum state to determine the antimicrobial activity of Aloe vera (Aloe vera) plant extract against Pseudomonas aeruginosa (P.aeruginosa ). The study was carried out on 100 bacterial isolates collected from different Khartoum hospitals in period from May to July 2019 . The powdered plant material was extracted by using 85% ethanol and different concentrations are (100, 75, 50, 25, 12.5 mg/ml) prepared from plant extract And the antimicrobial activity of Aloe vera was assayed in vitro by agar well diffusion method against P.aeruginosa isolates. P.aeruginosa isolates were collected from urine, wound, sputum, blood, ears, vagina, and ,pleural fluid, from patients with different ages. Conventional Biochemical techniques were used for isolates identification. Antibiotic discs were used for bacterial P.aeruginosa isolates sensitivity on Muller Hinton Agar (MHA). The tested antibiotic discs were Ciprofloxacin, Gentamicin, Ceftazidime, Imipenem, Meropenem and Amikacin. The concentration 100 mg/ml of the extract inhibited the growth of the all isolates 100(100%), while 75mg/ml inhibited 98(98 %) , 50mg/ml inhibited 75(75%), 25 mg/ml inhibited 18(18%) while the concentration 12.5mg/ml inhibited only 2 (2%) from total isolates. It is concluded from this study the different concentration of Aloe vera extract can inhibit the growth of P.aeruginosa.