Immunohistochemical Detection of Breast CancerType1 Mutant Gene and CancerAntigen125 in Ovarian Cancers among Sudanese Women

Abstract

This a descriptive retrospective case hospital based study was conducted in Radiation and Isotope Center Khartoum (RICK), and AL-Amal hospital during the period from March 2017 to April 2018.The study aimed to detect BRCA1 mutant gene and CA125 in ovarian cancer using immunohistochemistry. Thirty three paraffin embedded block previously diagnosed as ovarian cancer were collected. Samples included 23(69.7%) epithelial tumors, 9(27.3%) sex cord tumors, and 1(3.0%) germ cell tumors. The patient’s age range between 32and 65 years with mean age of 49 years, most of patients were more than 40 years, representing 26(78.8%) and the remaining 7(21.2%) patients were less than 40 years. All samples prepred by microarray, two sections for microarray of 3 micrometer thickness were cut from each paraffin block by rotary microtome and stained by immunohistochemical method (Indirect dextran polymer immune peroxidase technique) for detection of BRCA1 mutant gene and CA125. Data collected from patient’s files and results obtained were analyzed using SPSS computer program. Immunohistochemical expression of BRCA1 mutant gene was not detected in all samples, while CA125 positive expression was observed in 27/33samples, and negative in 6/33 samples. The positive result of CA125 in histopathologicl types of ovarian cancer revealed that, in epithelial tumor 5/18 samples, sex cord tumor 1/8 samples and germ cell tumor no samples, with insignificant correlation between CA125 expression and ovarian cancer subtypes (P-value 0.698). The relation between CA125 positive expression and grading of malignant tumor as showed that, grade 1 in 2/9 samples, grade II in 4/10 samples and grade III no positive sample, with insignificant correlation between CA125 expression and ovarian cancer grades (P-value 0.331).

This study concluded that there is no relation between BRCA1mutant gene expression and ovarian cancer, and no relation between CA125expression and histopathological types and grade.