Assessment of IronProfile, Hemoglobin Level and Reticulocyte Count Among Sudanese Dialysis Patients Receiving Erythropoietin Injection, Khartoum State

Abstract

Background: This was retrospective analytical case control studyconducted in hemodialysis patients in Bashaier hospital during the period of April 2019 to January 2020, aimed to total of sixty subject ( thirty dialysis patient and thirty healthy individual as control group) Objective: the aim of this study was to asses of iron Profile& reticulocyte count among Sudanese dialysis patients. Materials & Methods: in this case control study conducted in Bashaier hospital at Khartoum State – Sudan. Sixty subjects including: (thirty Dialysis patients at the study area who completed questionnaires and collecting data & thirty healthy individuals as control group). Serum total iron and TIBC concentrations were estimated by enzymatic method in fully Automated Biochemistry Analyzer (Mindray BS-380, china). Serum total ferritin concentrations were estimated by specific enzyme-linked immunosorbent assay (ELISA) kit method in fully Automated Biochemistry Analyzer (TOSOH AIA 2000, Japan). Hemoglobin level were estimated by automated hematology analyzer ( sysmex KX-N21) and manual reticulocyte count by normal light microscope. Results: In our study, Serum Iron Profile levels in Dialysis patients compared to control group. the mean serum level of total iron, TIBC, Transferrin saturation %, Ferritin, Hemoglobin g/dl and retic count in Dialysis patients respectively were (1.07±0.2, 1.33±0.4, 1.27±0.4, 1.80±0.4,1.40±0.4, 1.07±0.2), and in control group respectively were(1.47±0.5, 1.60±0.4, 1.03±0.1, 1.13±0.3,1.47±0.5, 1.33±0.4), and there was a significant decrease of serum level of total iron and Transferrin saturation in Dialysis patients compared to healthy individuals (p value=0.01, 0.00)and significant increase of TIBC, Ferritin and retic count in Dialysis patients compared to healthy individuals (p value=0.01, 0.00, 0.00) Discussion: -Iron deficiency and Anemia are frequent complications in chronically hemodialyzed patients, rHuEPO considered an effective medication in the treatments of anemia of CKD but presence of other side elements will interfere such as blood transfusion and IV iron. Conclusion: - Significant decrease of serum level of total iron and Transferrin saturationand significant increase of TIBC, Ferritin,Hemoglobin and retic count in Dialysis patients treated with EPO compared to healthy individuals