Detection of β-fibrinogen 455G/A Gene Mutation among Sudanese Patients with Deep Venous Thrombosis

Abstract

This is analytical a case control study conducted in Khartoum state during the period from Mars 2018 to September 2018, the study aimed to Detect the fibrinogen polymorphism G-455A in sample of Sudanese patient with deep vein thrombosis. Forty patients diagnosed with DVT as case and forty healthy volunteers as control were included in this study. Venous blood samples were collected in EDTA containers, then DNA was isolated from peripheral blood leucocytes and β fibrinogen G455A polymorphism was detected by PCR. The data obtained was analyzed using SPSS program version 16. The age of case group ranged between 20 and 90 years with mean age (43±18). Most of patients were first diagnosed by DVT between 20-40 years and the majority of them were female. β fibrinogen gene wild type was seen in 35(43.75%) among case group and 36(45%) among control group, while mutant gene was seen in 5(6.25%) among case and 4(5%) among control group. However, there was no significant statistical differences (P- value 0.723). β-fibrinogen 455G/A polymorphism was detected at 5 DVT patients (6.25%) all of them were homozygote (A/A) type, while in control group 3 out of 4 were heterozygote (3.75%) and 1 out of 4 was homozygote (1.25%). The difference did not Show the statistical significance (P-value=0.058). Out of 40 patients confirmed to had DVT, A allele frequency of β fibrinogen - 455G/A gene was 10(6.37%) and G allele frequency 70 (44.59%). While among control group A allele frequency was 5(3.18%) and G allele frequency 75 (47.77%). This result shows significant statistical differences (P-value=0.016). The study concluded that β-fibrinogen 455G/A polymorphism was not linked to an increased risk for DVT, and neither did the heterozygous (A\G) nor homozygous (A\A) carriers show an increased risk for DVT.