Association of Mythylenetetrahydrofolate Reductase C677T Polymorphism and Myocardial Infarction Risk among Sudanese patients in Khartoum State

Abstract

This was an analytical case-control study that has been carried out in Khartoum state from October 2018 to June 2019 aimed to investigate the association between Methylenetetrahydrofolate C677T polymorphism and myocardial infarction risk. Eighty subjects were recruited for this study, forty patients with a history of myocardial infarction aged between 27 to 80 years and forty healthy volunteers as a control group. 13 of patients were suffering from diabetes mellitus, 14 had hypertension, 3 of them had the two diseases and ten of them did not have any risk factor. Data wer collected from participants (cases and controls) directly by questionnaire, included age, gender, other diseases and family history for cases and age and gender for control subjects. 2.5 ml intravenous blood samples were collected from all participants in EDTA anticoagulant containers, genomic DNA was extracted by salting out method and the methylenetetrahydrofolate C677T polymorphism was analyzed using allele-specific polymerase chain reaction. Amplified fragments separated on 2% agarose gel stained with ethidium bromide and demonstrated by gel documentation system, which produced two single bands one at 124 bp represented C wild allele and the other one at 50 bp represented T the mutant allele. The data analyzed by computer program statistical package for social sciences SPSS, version 16. The results showed that the frequencies of CC, CT and TT genotypes within case group were 21 (52.5%), 17 (42.5 %) and 2 (5%) respectively, while this frequencies within control group were 25 (62%), 15 (37%) and 0 respectively. The relative risk for methylenetetrahydrofolate C677T polymorphism was calculated (0.9) by SPSS program (95%CI: 0.885 to 1.020), it was shown that there was no significant association between having this mutation and risk of MI occurrence. Methylenetetrahydrofolate C677T genotypes distribution showed no significant difference between the case group and the control group P. value (0.290). Also, there was no significant relationship between risk factors which patients suffer from in this study and methylenetetrahydrofolate C677T polymorphism. In conclusion, there was no significant association between Methylenetetrahydrofolate C677T polymorphism and myocardial infarction risk among Sudanese patients in Khartoum state.