Evaluation of Multi-locus Sequence Analysis of different Genes in Rhizobium-Legume incompatibility

Abstract

This study was performed to characterize TAL169 bacteria which is used widely as bio-inoculants through sequencing of 16SrRNA, nifH, nodC, recA, and glnII genes. DNA of the bacteria was extracted, PCR amplification of different genes and sequencing were done. The results indicated that TAL169 is Mesorhizobium with 99% identity according to 16SrRNA, recA and glnII genes sequences analysis. At the same time, nifH gene analysis indicated that it is Sinorhizobium with the same identity. The phylogeny analysis of all genes placed TAL169 bacterium in separate categories. The study concluded that there is no genetic relationship between TAL169 and Bradyrhizobium which used in inoculation trials in different regions in the world and was named as TAL169. That is why the majority of previous studies findings used this bacterium as Baradyrhizobium in bio-inoculation recorded no significant differences in the production of legumes inoculated. So using of this bacterium is a useless process. Accordingly, for successful bio-inoculation and production improvement in agriculture, before preparation of the bacterial inoculants identification of locally isolated Rhizobia by sequencing and analyzing of the above-mentioned genes or more housekeeping genes is recommended.