Evaluation of Factor V Leiden Mutation among Pregnant Women With Preeclampsia

Abstract

This is a case control study ,carried in Khartoum state during the period from April to March 2019 , aimed to evaluate of factor V Leiden mutation among pregnant women with preeclampsia in Omdurman Maternity Hospitals. The study included 100 Sudanese females 50 pregnant women with preeclampsia and 50 non pregnant women , their age ranged 18 – 49years. All volunteers were verbally informed about the study and their consent for participation was obtained.3 ml of EDTA venous blood sample was collected from each volunteer. Polymerase Chain Reaction (eppendorf, master cycler) was used for detection of factor V Leiden mutation . The data collected consist of demographic data of women included age , education level , history of chronic disease , number of gravidity , history of thrombosis , and history of abortion. 100% of women were not have mutantion in factor V. Data analyze by using SPSS version 16( Statistical Package for Social Sciences). Age divided into three age groups (< 20) ,( 20-35),and (>35 ) years, the most frequent one was 20-35 years , while the least one was < 20 years in case group. As like control group, with mean and STD (29.1+ 6.1) years. In case group Regarding to the educational level , 6% of women had illiterate ,36% had higher education level. Healthy women were highest frequency compared with women have hypertension (80% , 20%) respectively, most of them (18%) use Nifedipine and 2% use Amilo5. According to gravidity high frequency was primy gravidity 32%, followed by secondy gravidity and grand multi gravity 28% and then the multi gravidity 12% . Eighty six of women not have thrombosis history but 14 % of them have history of thrombosis , regard to the abortion 40% have history of abortion and 60 % not have history , high frequency of abortion number is one time 22%, two time 14% and three time 4% . The results showed absence of gene mutation in both case and control, with no statistically significant difference in factor V between pregnant and non pregnant women. Also there was no significant association between mutation and demographic data , as well as mean of age between cases and control p.value (p=0.56).