Immunohistochemical Detection of Laminin Protein in Sudanese Women with Breast Tumors

Abstract

This is hospital based descriptive retrospective case study conducted in Omdurman Teaching Hospital in Khartoum state, during the period from December 2018 to March 2019. The study aimed to detect the expression of laminin in breast tumors using immunohistochemical method. Forty paraffin embedded blocks from patients samples previously diagnosed as breast tumors were collected. One section of 3 micron thickness was cut from each paraffin block by rotary microtome and stained by immunohistochemical method (avidin biotin technique) for detection of laminin. Data collected from patient's files and results were analyzed using SPSS computer program. Samples include 20 (50%) samples were malignant tumors, including invasive ductal carcinoma 16 (40%) samples, lobular carcinoma 2(5%) samples, micro papillary carcinoma 1 (2.5%) sample and metaplastic squamous cell carcinoma 1(2.5%) sample. And 20 (50%) samples were benign tumors; all of them were fibroadenoma samples. Grade of malignant tumors were 1 (5%)sample was grade 1, 9 (45%) samples were grade 11, 6 (30%) samples were grade 111, 4 (20%) were not graded. The patients age range between 18 to 90 years with mean age of 40 years. Malignant breast tumors revealed strong positive expression of laminin in 7 (35%) samples, and weak positive in 13 (65%) samples, while all benign tumors showed strong positive expression of laminin with significant association (P=0.000). This study concludes that breast tumor tissue express laminin with high expression in benign tumors.