Association of PT, APTT, Platelets count and Fibrinogen level with Type I and Type II Diabetes Mellitus Patients in Khartoum State

Abstract

Background: The hyperglycemia in diabetic mellitus patient will result in oxidative stress that cause the generation of free radicals, glycation and advanced glycation end products which leads to platelet abnormalities, increased levels of coagulation factors and enhanced fibrinolytic activity, these changes might lead to coagulation disorders in those patients. The aim of this research is to study the coagulation parameters in type I and type II diabetes mellitus patients. Methods: This was Non interventional analytical case control study, done on a period of three months from November 2017 to January 2018 at Aliaa specialist hospital. One hundred diabetic patients (50 with type 1 and 50 with type 2 diabetes mellitus) were enrolled in the study. Fifty apparently healthy non-diabetic subjects were selected as a control group. The blood samples were collected from cases and controls, 1.8 ml of blood in Tri Sodium Citrate tube for measuring the coagulation parameters: (PT, APTT and Fibrinogen level) by using Helena AC-4 and 3 ml of blood in EDTA K3 for platelets count by using Sysmex XP-300 analyzer and 3 ml of blood also in Sodium Floride for measuring the FBS by using Cobas Integra 400 plus. Data were analyzed by using statistical package of social science (SPSS) program. Results: The results obtained from this study that males were (52.7 %) and females were (47.3%). PT in the case group was significantly decreased (P value = 0.0078) and also in DM type I (P value = < 0.001). APTT in the case group was significantly decreased (P value = 0.0190) and also in DM type I (P value = 0.0187). Platelets count in the case group was insignificant (P value = 0.2902) and in DM type I was significantly high (P value = 0.0172). Fibrinogen level in the case group was significantly high (P value = <0.001) and also in the DM type I (P value = 0.0030). There were no statistically significant differences in all measured parameters with duration of DM (P value = > 0.05), also with gender no significant differences (P value = > 0.05). Conclusion: Type I DM patients were more affected than type II. Diabetic patients were subjected to haemostatic abnormalities, accordingly routine coagulation tests are recommended from time to time for better management of DM.