Evaluation of Plasma Interleukin-8 Levels among Sudanese Hepatitis B Patients in Khartoum

Abstract

One of the most important factors playing role in hepatitis B pathogenesis is cytokine release and the most effective cytokines that with proinflammatory characteristic is interleukin-8 (IL-8). This is a case-control study was aimed to evaluate the IL-8 level in Sudanese hepatitis B cases and in control subjects in Khartoum state during the period from March to September (2019). Sixty subjects, selected randomly in this study, with age varies from 12-50 years, 30 subjects were hepatitis B patients (17 male and 13 female) as case group and 30 subjects (17 male and 13 female ) were age and sex matched healthy control group. Venous blood sample (3ml) was collected in EDTA container from each subject. IL-8 concentration was measured using Enzyme Linked Immunosorbent Assay (ELISA) in Ibn Sina University laboratory. The data was analyzed using Statistical Package Social Science programme (Version 20). Students T test and One way ANOVA test were used to compare between means. P. value significant when ≤0.05. Means of IL-8 were 43.11 ±30.01, 28.8±22.8 in the case group and control group respectively. IL-8 level was significantly elevated in hepatitis B patients than the and control group (P. values 0.042). Also there was no statistical correlation between IL-8 and age in case and control groups (P. value 0.524 and 0.994 respectively). The difference in mean of IL-8 between males and females of the case group and control group was not statistically significant (P. value 0.334 and 0.341 respectively). The difference in mean level of IL-8 in case group for selected variables(Duration, Treatment, Blood transfusion and jaundice) was no statistically significant (P. values 0.627, 0.982, 0.486 and0.339 respectively). The study concluded that in the studied population, IL-8 concentration may be useful as prognostic marker for hepatitis B infection.